-˽SɫcBSEBԙzyԇ
| (yng)N | |
| zy | ж |
| ԇеĸҺwM֑(yng)oװX(yng)հboƓp© | |
| ; | ڶԙzyѪѪ{M{P(gun)Һwӱ˽SɫcBSEB |
| ԭ | ԇвgӷø(lin)ԇELISAA(y)Ȱ˽SɫcBSEB@ԭİУμ(bio)ԺԌգټHRP(bio)ӛęzyw(jng)^صϴõTMB@ɫTMB^øĴD(zhun){ɫD(zhun)KSɫɫ\͘Ʒе˽SɫcBSEBP(gun)ø(bio)x450nm LyȣODֵжԡ |
| خ | / |
| ؏(f) | (ni)׃ϵ(sh)С15% |
| / | |
| ԇԂԇ | 1ø(bio)x450nm2߾ȼӘ^0.5-10uL2-20uL20-200uL200-1000uL3374sˮȥxˮ |
| 1Ҝƽ 20min XȡlʣlԷܷŻ 42O(sh)ԌտԌտ͘ӱףԌտ 50L Ʒӱмyӱ 50Lհײӡ3հտ͘ӱÿ^øHRP(bio)ӛęzyw 100L÷Ĥס(yng)37ˮԡ偻 60min4ȥҺwˮĸÿӝMϴҺ350Lo 1min˦ȥϴҺˮĸ؏(f)ϴ 5 ΣҲϴCϴ壩5ÿ AB 50L37ܹ 15min6ÿKֹҺ 50L15min (ni) 450nm L̎y OD ֵ | |
| ע | 1Ҏ(gu)ĕrg͜ضMМԱC(zhn)_Y(ji)ԇʹǰ_Ҝ 20-25档ʹúرԇ2ϴ岻_Ԍ(do)²(zhn)_ĽY(ji)ڼǰ_Mɿ׃(ni)Һw^вҪ3ךҺwָӡtӰ OD ֵ4@ɫҺ(yng)ʟoɫܜ\ɫѽ(jng)׃{ĵҺʹ5ԇ͘(bio)ĽȾe`Y(ji)6ڃ͜r⏊ֱ䡣7ƽҜغٴ_ܷԷˮl8κη(yng)ԇܽ|Ư |
| } | ن}(bio)(zhn)ԭ(yng)ߣ1.ԇƽrg 2 СrҜƽ2.ȡϴ첻ֵ֡ȡϴ3.Һ_zУҺچ}ܶȵͿԭ(yng)ߣ1.ϴ첻֡fҪϴͽ2.ֺȡԇ㡪ֻȡԇ3.؏(f)^ĤʹüӘҪQµ^ʹµķģ4.ӘӲ_zУҺۆ}O.DֵͿԭ(yng)ߣ1.ÿӵԇ_УҺ_ԇ2.rg_CĜrg3.ضȲ_ԇҪƽҜزC(zhn)_Ĝض4.ø(bio)ӛʧЧͨ^ø(bio)ӛ͵ɫѸ@F(xin)z5.]мKֹҺfEKֹҺ6.x(sh)rgx(sh)f]x(sh)rg(ni)x(sh)܆}ӱֵԭ(yng)ߣ1._Ęӱ淽ʽ_ӱʹrӱMЌ2._Ęӱռ̎ȡ_Ęӱռ̎3.y|(zh)ژӱк͡ʹrӱ؏(f) |
| | ԓԇЃHоʹ猢R\κ;ҹ˾ˮa(chn)Ć}ؓ؟(z)Г(dn)κη؟(z) |
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(qun)УȪƼ˾ | }ICP18019542̖ | g(sh)֧֣xW(wng)
